ʻO UV-Vis Spectroophotometers he mau mea akamai i hoʻohana nuiʻia i nā meaʻepekema i hoʻohanaʻia ma nā kahuaʻepekema. Me ka nui o ko lākou mea nui,ʻaʻole nā kānaka he nui Hāʻawi kēiaʻatikala i nā wehewehe i ka wehewehe hohonu o UV-Vacksrophoderter, e uhi ana i ko lākou mau kumu, hoʻohana i ko lākou hana.
He aha kahi mea i loaʻa iā UV-Vis Spectrophotometer?
ʻO kahi Spectrophotomet UV-Vis Spectrophotomet he mea pono i hoʻohanaʻia e ana i keʻano o ka nui o ka mālamalama i ka pae koho electroagnetic. He koʻikoʻi kēia mau mea kani no ka nānāʻana i nā waiwai optical o nā mea i loaʻa, a hoʻomaopopo i ko lākouʻano.
Pehea e hana ai kahi mea hana UV-Vis.com?
ʻO ka hana o kahi Spectrophotometer UV-Vis Spectropholdometer e pili ana i kekahi mau kumu nui a me nā hana:
Nā Kūlana Kūʻai:
Aia ka Spectrophotometer i kahi kumu kukui, maʻamau i ka huiʻana o kahi kukui deuterium (no ke kukui uv ʻO kēia mau kukui kukui e emi i waena o ka UV a me ka nānāʻana.
Monochromator:
ʻO ka mālamalama e hoʻoneʻeʻia e ke kumu e hele ai ma kahi monochromator, e hoʻokaʻawale ana iā ia i loko o nā manawa lōʻihi. Hana pinepineʻia kēia me ka hoʻohanaʻana i kahi prism a iʻole kaʻohiʻana.
SHORPLE DONE:
Ua kuhikuhiʻia ka mālamalama monochromatic ma o ka spetple bearter, kahi i kauʻia ai ka spele speple i loko o kahi cuvette, he pahu liʻiliʻi i hanaʻia i ke aniani a iʻole kahi pahu liʻiliʻi.
Uʻa Uʻi:
A pau ma hope o ka paukū, ua hiki hoʻi ka mālamalama i kahi mea hopu. Keʻae nei ka mea hana i ka ikaika o ka mālamalama i laweʻia a hoʻohuli iā ia i loko o kahi hōʻailona uila.
Nā Manaʻo Pūnaewele:
ʻO ka hōʻailona uila e hōʻikeʻia e kahi kamepiula a microprocessor, kahi i hoʻohiolo ai i kahi mea hoʻopiʻi e hōʻike ana i kaʻano likeʻole o ka nūhou.
Nā loina o UV-Vis Spectroophotry
ʻO ke kumu kumu kumuʻauhau ma hope o ka victorroptotort i pā'āluaʻo UV-VISH i ke kānāwai karala, e pili ana i keʻano o ka mālamalama o nā mea i hele ka māmā. Hōʻikeʻia ke kānāwai:
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hea:
ʻO kahi o kaʻaeʻana
ʻO ka molar bodorpttivity coirorttivity
ʻo ia kaʻike o nāʻano o ka laweʻana i nāʻano likeʻole i ka sample (mol / l).
ʻo ke ala lōʻihi e hele ai ka mālamalama i ka sample (cm).
ʻO ka hoʻopiliʻana i kaʻaeʻana a me ka lōʻihi o ke komoʻana a me ke ala, e hana ana i nā mea hana UV-Vis
Nā noi o UV-Vis Spectrophotometers
ʻO UV-Vis SpectroophotomTomTometer i kahi ākea nui o nā noi ma waena o nā papa likeʻole:
1. Kemiki
Hoʻoholo i ka manaʻo hoʻoholo:
ʻO UV-Vis Spectroophotomet maʻamau e hoʻohana pinepineʻia e hoʻoholo i keʻano o nā mea i loaʻa i kahi hopena. No ka manawaʻike, kaʻaha hoʻohui o Dna, nā polokalamu uila, hiki ke ana i nā anal
Nā kila i keʻano:
Kōkua kēia mau mea i ke aʻoʻana i nā kumukūʻai o nā pono o ke kahe e ka nānāʻana i ka hoʻololiʻana i nā mea hana a iʻole nā huahana i ka manawa.
Kekuhi Kūʻai:
Hoʻohanaʻia lākou no ka helu helu helu a me ka helu heluʻana o nā mea kanu lāʻau, kōkua i nā mea e pili ana i kā lākou mea e pili ana i kā lākou mea e pili ana i kā lākou mea e pili ana i kā lākou mea e kau nei
2. Biochemistry a me Molecular Biology
ʻO ka protein a me ka nui o ka nui o ka nui o ka nui:
ʻO UV-Vis SpectrophotyTyyyyyyyyy
ʻO ka hana enzyme:
Hiki ke aʻoʻia ka hana o nā enzymes e ana ma ke anaʻana i kaʻaeʻana o nā mea hoʻopiʻi a iʻole nā huahana e pili ana i nā hopena enzymatic.
3,ʻepekema kūlohelohe
ʻO ka ho'āʻoʻana i ka wai:
Ua hoʻohanaʻia nā mea nānā UV-VISA iʻikeʻia eʻike a hoʻomaka i nā mea Health i ka wai, e like me nā pālani, a me nā phoptas, a me nā kī nui.
E nānā ka ea maikaʻi:
Kōkua lākou i ka nānāʻana i nā polu ea o ka ea e ka anaʻana i ka hoʻopiliʻana i ka piliʻana o nā kino e like me ka ozone a me nitrogen dioxide.
4. E nānā a me ka loiloi lāʻau lapaʻau
ʻO ka ho'āʻoʻana a me ka hoʻomohalaʻana:
Ma kaʻoihana lāʻau lapaʻau,ʻo UV-Vis Spectroophotometer e hoʻohanaʻia e hoʻopaʻa i kaʻike a me ka hoʻomaʻamaʻaʻana i nā lāʻau lapaʻau a me ka degradation o nā lāʻau lapaʻau.
Nā Kūlana Kūʻai:
Kōkua kēia mau mea aʻoaʻo i nā maʻi maʻi lapaʻau ma ke anaʻana i ka hoʻopiliʻana o nā mea likeʻole, e like me ka glucose
5.ʻO ka meaʻai a me nāʻoihana hana
Kākau pono:
Hoʻohanaʻiaʻo UV-Vis Spectrophotometyy
ʻO ka nānāʻana o ka nutritional:
ʻO ka hoʻohālikelike o nā vitamins, nā minerala, a me nā meaʻai'ē aʻe i nā huahana meaʻai e hiki ke hoʻoholo i kēiaʻano hana.
Nāʻano o ka UV-Vis Spectrophotometers
Uv-Vis Spectropholdomet CO
I loko o nāʻano hana likeʻole, i kēlā me kēia mea i kūpono no nā noi kiko'ī.
Smet-Beam Spectrophortoters:
Loaʻa iā lākou kahi ala māmā hoʻokahi, keʻano o ka'ōlelo a me nā ana likeʻole. He maʻalahi lākou aʻoi aku ka nui o ka uku-ʻoi aku ka maikaʻi akā hiki ke emi iki ma muli o nā loli kūpono ma keʻano o ke kino māmā.
Nā Spectrophotomsomsomerʻelua:
Hoʻopili kēia mau mea kani i ka mālamalama i nā alaʻelua, hoʻokahi e hele ana ma o ka laʻana a me nā mea'ē aʻe. Hāʻawi kēia hoʻonohonoho i ke anaʻana me ka ukuʻana, e uku ana no nā loli i ka mālamalama a me ka hāʻawiʻana i nā hopena kūpono.
Nā mea heluhelu microplat:
Hoʻonohonohoʻia no ka hoʻomohala kiʻekiʻe, hiki i nā mea heluhelu Microplay ke ana i nā hana likeʻole me ka monipokano nui me nāʻoihana Biotechnical.
PRITATATATATATATATATATAS UV-VIS SPECTROTHORTOMETERS:
ʻO kēia mau mea hoʻohālikelike, ua hoʻohanaʻia nā polokalamu lima i hanaʻia no ka hanaʻana a me ka loiloi pūnaewele, e hāʻawi ana i kaʻoluʻolu a me ka maʻalahi o ka mālamaʻana i keʻano o ke kaiāulu.
Nāʻenehana holomua a me nāʻano likeʻole
Uv-Vis Spectroophotometry ua ulu houʻia e hoʻokomo i nāʻenehana holomua a me nā loli:
1
Hoʻokomoʻia kēiaʻano hana i ka hoʻololiʻana i ka spectrinance spectrinance, e hoʻonui i ka hopena o nā peaks e hoʻohuli ana i nāʻano hoʻohālikelike.
2, pani-ahi
Ua hoʻohanaʻia e aʻo i nā kinipōpō kīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwīwī KOLOHE ANA
3
ʻO ke kumu o kēiaʻoihana ke hanaʻia e ka hoʻokahe o ka molūʻana, e hāʻawi pono i kaʻano o ka hoʻopaʻaʻana iāʻikepili UV-VIS
Loaʻa a me nā palena
Loaʻa
ʻAʻole hana hewaʻole:
Uv-Vis Spectrophotomet he mea maʻamauʻole ka hana hewaʻole, mālama i ka hōʻike no ka nānā houʻana.
ʻO ka noʻonoʻo kiʻekiʻe a me ka pololei:
Hāʻawi kaʻenehana i keʻano kiʻekiʻe a me ka pololei, e kūpono ana no ka nānāʻana a me ka heluʻana i nā haʻahaʻa haʻahaʻa haʻahaʻa.
Kahua:
Hiki iā ia ke nānā i kahi ākea nui o nā mea waiwai, me nā mea hui a me nā hui pū a me nā mea komoʻole.
Rapid a me ka maʻalahi:
ʻO nā ana a me nā pae wikiwiki a pololei hoʻi, eʻaeʻia no ka hoʻohana ponoʻana a me ka loiloi maʻamau.
PAHUI
Nā Kūlana:
ʻO ka heleʻana o nā mea hoʻohālikelike e pili ana i nā mea e hoʻopili ai i nā mea likeʻole e hiki ke hōʻemi i ka loiloi.
Mākaukau hoʻomākaukau:
Hiki i kekahi mau hiʻohiʻona ke koi i ka hoʻomākaukau nui a iʻole ka hanaʻana, e hōʻike ana i nā halaʻole.
ʻO kaʻike pili:
Ua hāʻawiʻo UV-Vis Spectroophotomet
ʻO UV-Vis Spectroophotometers he mau mea hana e pono ai i kaʻepekema hou, e hāʻawi ana i kahiʻano hana ikaika a me keʻano ikaika no ka hoʻopaʻaʻana i kahiʻano nui. ʻO kā lākou mau noi pili i keʻano o nā kīhāpai, me nā chemistry, biochemistry,ʻepekema diagnostics, a me kaʻoihana meaʻai. Hoʻomaopopo i nā loina, ka hana, a me nā hoʻohana o UV-Vis Spectroophotry a hiki i nā loea a me nā hana hana. ʻOiaiʻo kāna mau palena, ka mea kūʻai akuʻo UV-VIS-VIS
