UV-Vis Spectrophottometers yog cov twj paj nruag ntau siv nyob rau ntau qhov kev tshawb fawb. Txawm hais tias lawv tseem ceeb, ntau tus neeg tsis nkag siab cov khoom siv no yog, lawv cov kev siv, thiab lawv ua haujlwm li cas. Kab lus no aims los qhia txog UV-Vis Spectrophotometers, npog lawv cov ntsiab lus, siv, thiab cov xwm txheej uas lawv ua haujlwm.
Dab tsi yog UV-Vis Spectrophotometer?
Tus UV-Vis Spectrophotiver yog ib qho kev tshawb xyuas cov cuab yeej siv los ntsuas cov kev siv ntawm lub teeb hauv lub teeb (UV) thiab vis) ntawm lub electromagnetic spectrum. Cov twj paj nruag no yog qhov tseem ceeb rau kev txheeb xyuas qhov muag kho qhov muag ntawm cov tshuaj, txiav txim siab lawv tus cwj pwm nyob hauv kev sib txawv.
UV-Vis Spectrophotoma ua haujlwm li cas?
Kev ua haujlwm ntawm UV-Vis Spectrophotometer muaj ntau cov ntsiab lus tseem ceeb thiab cov kauj ruam:
Qhov Chaw Teeb:
Lub spectrophottomotometer muaj lub teeb ci, feem ntau yog kev sib xyaw ntawm kev teeb ci (rau UV teeb) thiab lub teeb tungsten (rau pom lub teeb). Cov teeb no tshwm sim ntawm lub UV thiab pom pom Spectra.
Monochromator:
Lub teeb emitted los ntawm lub hauv paus hla los ntawm moob, uas cais nws mus rau hauv ib tus neeg wavelengths. Qhov no feem ntau yog ua tiav siv prism lossis txawv grating.
Tus Qauv Nrog:
Monochromatic lub teeb yog qhia los ntawm tus qauv kev daws teeb meem, qhov twg cov qauv kev daws teeb rau hauv ib lub cuvette, lub thawv me me ua los ntawm iav lossis quartz.
Ntes:
Tom qab dhau los ntawm cov qauv, lub teeb mus txog ib qho ntes. Cov ntsuas ntsuas ntsuas qhov siv ntawm lub teeb sib txuas thiab hloov nws mus rau hauv lub cim hluav taws xob.
Cov ntaub ntawv tsom xam:
Lub ntsuas hluav taws xob yog tom qab ntawd ua tiav los ntawm lub khoos phis tawj lossis microprocessor, uas tsim cov spectrum uas ua rau cov qauv nqus lossis kev hloov pauv ntawm cov wavelength.
Cov hauv paus ntsiab lus ntawm UV-Vis Spectrophotometry
Lub hauv paus ntsiab lus tseem ceeb tom qab UV-Vis Spectrophotometry yog cov npias-Lambert txoj cai, uas cuam tshuam kev nqus ntawm lub teeb ci rau cov khoom uas lub teeb tau mus ncig ua si. Txoj cai yog qhia raws li:
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Qhov twg:
A yog qhov nqus tau (tsis muaj units, vim nws yog qhov sib piv).
Yog tus mob Molar daws cov coeffptivity coeffinentity (L / Mol cm), ib qho tsis tu ncua uas qhia tias lub teeb pom kev zoo li cas ntawm ib qho yoj.
yog qhov concentration ntawm nqus cov hom hauv cov hnoos qeev (mol / l).
yog txoj kev ntev los ntawm qhov teeb tsa taug kev hauv cov hnoos qeev (cm).
Absorbance is directly proportional to the concentration and path length, making UV-Vis spectrophotometry a powerful tool for quantitative analysis.
Cov ntawv thov ntawm UV-Vis Spectrophottometers
UV-Vis Spectrophottometers muaj ntau cov ntawv thov hla cov teb sib txawv:
1. Chemistry
Kev txiav txim siab txog kev txiav txim siab:
UV-Vis Spectrophottometers yog cov niaj hnub siv los txiav txim siab ntawm kev sib haum xeeb ntawm kev daws teeb meem hauv kev daws teeb meem. Piv txwv li, kev pom zoo ntawm DNA, cov protein, lossis lwm cov biomolecules tuaj yeem ntsuas los ntawm lawv cov kev nqus ntawm cov wavelengths.
Cov tshuaj tiv thaiv kinetics:
Cov cuab yeej kev pab hauv kev kawm cov nqi ntawm cov tshuaj tiv thaiv tshuaj los ntawm kev saib xyuas cov kev hloov pauv ntawm reactants lossis cov khoom lag luam dhau sijhawm.
Tshuaj Ntsuam Xyuas Tshuaj:
Lawv tau siv rau cov kev tshuaj xyuas cov qauv thiab kev txheeb xyuas kom muaj tshuaj lom neeg, pab txheeb xyuas cov tshuaj ntawm lawv qhov nqus tau.
2. Biochemistry thiab molecular biology
Protein thiab nucleic acid animfula:
UV-Vis Spectrophotometry yog qhov tseem ceeb hauv biochemistry rau kev ntsuas cov kev ntsuas thiab huv ntawm nucleic acids (DNA thiab Rau) thiab cov protein.
Enzyme kev ua:
Cov kev ua si ntawm cov enzymes tuaj yeem kawm los ntawm ntsuas ntsuas qhov nqus ntawm substrates los yog cov khoom lag luam cuam tshuam nrog enzymatic.
3. Ib puag ncig science
Dej Ua Zoo Kuaj:
UV-Vis Spectrophottometers yog siv los tshawb xyuas thiab kom muaj kuab paug hauv dej, xws li nitrates, phosphates, thiab hlau hnyav.
Cua Saib Xyuas Kev Zoo:
Lawv pab hauv kev soj ntsuam cov pa huab cua los ntawm kev ntsuas cov pa roj nqus ntawm cov roj cua zoo li ozone thiab nitrogen dioxide.
4. Cov tshuaj ntsuam thiab tshuaj tsom xam
Kev Ntsuas Tshuaj thiab Kev Loj Hlob:
Hauv kev lag luam kws tshuaj, UV-Vis Spectrophottometers yog siv los tshuaj xyuas cov kev xav thiab huv ntawm cov tshuaj thiab kev kawm ruaj khov thiab kev poob qis ntawm cov tshuaj sib xyaw.
Kho mob kuaj mob:
Cov twj paj nruag no pab hauv kev kuaj mob kuaj mob los ntawm kev ntsuas cov tshuaj ntawm ntau yam hauv lub cev nqaij hauv lub cev, xws li cov piam thaj, thiab bilirubin.
5. Khoom noj khoom haus thiab dej haus
Tswjhwm Zoo:
UV-Vis Spectrophotometry yog siv los xyuas kom zoo thiab kev nyab xeeb ntawm cov zaub mov thiab dej haus los ntawm kev ntsuas cov kev sib ntxiv, cov tshuaj tiv thaiv cov tshuaj ntsuab, thiab paug.
Kev noj haus tshuaj ntsuam:
Cov kev xav ntawm cov vitamins, minerals, thiab lwm yam zaub mov hauv cov khoom noj khoom noj tuaj yeem txiav txim siab siv cov txheej txheem no.
Hom UV-Vis Spectrophottometres
UV-VI SPECTROPHOTOMERERS CO
Kuv nyob rau ntau qhov kev teeb tsa, txhua tus raug rau cov ntawv thov tshwj xeeb:
Ib Leeg-Beam Spectrophhottometers:
Cov no muaj ib txoj hauv kev lub teeb, lub ntsiab lus ntawm cov kev ntsuas thiab ntsuas ntsuas yog coj ua ntu zus. Lawv yooj yim dua thiab muaj txiaj ntsig zoo dua tab sis tuaj yeem tsis tshua yog vim muaj peev xwm ua tau zoo nyob rau hauv kev siv teeb.
Muab ob npaug rau-nqaj spectrophottometers:
Cov twj siv no faib lub teeb ua ob txoj kev, ib qho dhau los ntawm cov qauv thiab lwm yam los ntawm kev siv. Cov teeb tsa no tso cai rau kev ntsuas kev ntsuas, them nyiaj rau kev hloov pauv hauv lub teeb siv thiab muab cov txiaj ntsig tau ntau dua.
Micplate nyeem ntawv:
Tsim los rau high-topple tshem tawm kev kuaj xyuas, microplate cov neeg nyeem tuaj yeem siv cov hnoos qeev ib zaug, siv ntau hauv kev lag luam biotechnology thiab tshuaj lag luam.
Portable UV-Vis Spectrophottometers:
Cov khoom siv no, Handheld cov khoom siv tau siv rau kev ua haujlwm thiab cov khoom siv ntawm sab xaib, muab kev yooj yim rau kev saib xyuas ib puag ncig thiab kev tswj hwm ib puag ncig.
Cov txuj ci siab thiab kev hloov pauv
UV-Vis Spectrophotometry tau hloov pauv kom suav cov txuj ci siab thiab hloov pauv siab:
1. Derivative spectrophotometry
Cov txheej txheem no suav nrog kev xam pom kev ua kom haum, txhim kho qhov kev daws teeb meem ntawm kev sib tw ntsuas ntawm kev ntsuas siab hauv cov mixtures.
2. Nres-ntws spectrophotometry
Siv los kawm cov tshuaj tiv thaiv sai sai kinetics, nres-ntws spectrophotometry sib xyaw rau hauv lub sijhawm-sijhawm, muab cov kev pom sai sai thiab ntsuas cov txheej txheem sai thiab tshuaj lom neeg.
3. Yees duab spectroscopy
Cov qauv no ntsuas cov suab nrov uas tau tsim los ntawm kev nqus ntawm lub teeb pom kev zoo, muab cov qauv siab rau kev kawm cov khoom siv khov kho thiab oV-vis spectrophottometry tej zaum yuav tsis muaj txiaj ntsig.
Qhov zoo thiab cov kev txwv
Qhov Zoo
Tsis yog-ua kev puas tsuaj:
UV-Vis Spectrophotometry feem ntau tsis yog kev rhuav tshem, khaws cov qauv rau kev txheeb xyuas ntxiv.
High rhiab thiab precision:
Cov txheej txheem no muaj cov siab rhiab thiab ua kom haum, ua rau nws tsim nyog rau kev kuaj pom thiab ntau yam kev ntsuas qis ntawm cov analytes.
Versatility:
Nws tuaj yeem tshawb xyuas ntau hom tshuaj, nrog rau cov organic thiab inorganic tebchaw, hauv ntau lub xeev (khoom, kua, thiab roj).
Sai thiab yooj yim:
Kev ntsuas ntsuas feem ntau yog nrawm thiab ncaj, tso cai rau kev tshuaj ntsuam xyuas tau zoo thiab ua ntu zus.
Kev txwv
Kev Cuam Tshuam:
Lub xub ntiag ntawm kev cuam tshuam cov tshuaj uas nqus ntawm wavelengths zoo sib xws tuaj yeem ua rau kev tsom xam.
Kev Npaj Qauv:
Qee qhov qauv yuav xav tau kev npaj ua kom ntev los yog dilution, muaj feem tau qhia txog kev ua yuam kev.
Cov ntaub ntawv tsawg:
UV-Vis Spectrophhotometry feem ntau muab cov ntaub ntawv ntawm kev pom cov ntsiab lus pom, uas yuav tsum tau ua cov txheej txheem sib txuas ntxiv xws li cov khoom siv roj ntau lossis NMR.
UV-Vis Spectrophotometers yog cov cuab yeej tsis tseem ceeb hauv kev tshawb fawb niaj hnub, muab ib qho txuj ci thiab muaj zog thiab muaj zog rau kev txheeb xyuas cov tshuaj dav dav. Lawv cov ntawv thov nyob hauv ntau thaj chaw, suav nrog chemistry, biochemistry, ib puag ncig kev tshawb fawb, chaw kuaj mob, thiab kev lag luam khoom noj. Nkag siab txog cov qauv, kev ua haujlwm, thiab siv UV-Vis Spectrophotometry tso cai rau kev tshawb fawb thiab kev tswj hwm, thiab kev tswj hwm kom zoo, thiab kev tswj hwm zoo, thiab kev tswj hwm zoo thiab ntsuas kev ua haujlwm. Txawm hais tias nws cov kev txwv, UV-Vis Spectroptomotometer tseem yog lub hauv paus ntawm testical lub chaw soj ntsuam, pab txhawb kom nce qib hauv kev tshawb fawb thiab thev naus laus zis.
