What is feature of our Western Blotting Transfer System?
WCL-212wet protein transfer system is a highly efficient western blotting transfer system that uses a novel technology (patent pending) developed by GenScript. WCL-212 combines the high reproducibility and efficiency of the traditional wet transfer with the speed and convenience of other fast transfer systems. The device allows efficient blot transfer of small, medium and large molecular weight proteins within 9-17 minutes.
Features of WCL-212 wet protein transfer system
Comprehensive: Outstanding performance for small, medium and large proteins
Convenience: Simultaneously transfer two mini gels
Compatible: Highly compatible with different types of precast or homemade gels
Simple: Easy to assemble, one button operation and customizable programs available
The WCL-212 system is for wet electroblotting of proteins from mini gels onto PVDF or nitrocellulose membranes. Enjoy a top-quality and rapid protein transfer WCL-212 brings you!
Embrace the brand new experience that WCL-212 brings you!
Why choose Western Blootting Transfer System ?
Conventional wet transfers are cumbersome, require experience and a great deal of time spent on consumable preparation. The transfer times usually vary from 1 hour to overnight. With eBlot fast wet transfer system, achieve excellent transfer efficiency without sacrificing speed.
WCL-212 allows fast and efficient transfer of 1 or 2 mini gels at a time. The setup takes less than 2 minutes and avoids the mess of a traditional wet transfer with our easy to assemble dry sponge sandwich
Our customizable protocols allows optimal flexibility for your research. The unit comes with standard protocols designed to transfer larger proteins (up to 400kD) or opt for a shorter transfer time for smaller size proteins (up to 100kD).
EGFR (170 KD; from left to right: 20 µg, 10µg, 5µg, 2.5 µg, 1.25 µg)
Taq (93 KD; from left to right: 125 ng, 62.5 ng, 31.2 ng, 15.6 ng, 7.8 ng)
GAPDH (36 KD; from left to right: 10 µg, 5 µg, 2.5 µg, 1.25 µg, 0.25 µg)
Comparative blotting efficiency with various transfer systems. Three different proteins were transferred on to PVDF membranes using traditional wet transfer, iBlot, Trans-Blot, and GenScript's WCL-212. The quantities of proteins loaded ranged from 1.25 µg- 20 µg for EGFR, 7.8 ng- 125 ng for Taq, and 0.6 µg- 10µg for GAPDH. For all three proteins, the WCL-212 offered superior transfer efficiency compared to other systems. With its high efficiency of transfer, protein concentrations as low as 7 ng for Taq (lane 5), and as high as 20 µg for EGFR(lane 1) could be clearly visualized.