I. Sumo
Kuunganidzwa kweropa kwakakosha zvishandiso zvakakosha mumarabhorendi, kufambisa muunganidzwa, kuchengetedzwa, uye kugadzirisa kweropa samples dzekuongorora. Kusarudzwa kwakakodzera uye kushandiswa kweaya machubhu akakosha kuti uwane kuwana mhinduro dzakarurama uye dzakavimbika, izvo zvinoita basa rakakosha mukuongororwa kwemurwere uye manejimendi.
Ii. Mhando dzakajairika dzeropa dzekuunganidza ropa
A. Serum inoparadzanisa machubhu (sst)
Serum inoparadzanisa machubhu, inowanzozivikanwa semasita, akagadzirirwa kufambisa kuparadzaniswa kweSerum kubva kuRopa rese mushure mekunge centrifugation. Aya machubhu ane jege anoparadzanisa, kazhinji akagadzirwa neinert zvinhu senge silicone kana silica, yakamiswa pakati peiyo clot activator uye serum. Munguva yezana, gel inoumba chipinganidzo pakati peSerum uye clot, ichibvumira kupatsanurwa kwakachena. SSTS iri kushandiswa zvakanyanya kune akasiyana emapiritsi emakomduketi, kusanganisira yeropa basa bvunzo, lipid profiles, mahormoni assays, uye hutachiona hwechirwere chemitambo.
B. Ethylynediamaminyacetactic acid (Edta) machubhu
Edta machubhu ane anticoagulants ethyleentiamailamailasetraactic acid, iyo inosungira calcium ion muropa uye inodzivirira coagulation nekunyadzisa kuita kwezviitiko zvecogerlines. Aya machubhu anonyanya kushandiswa kune hematologicological kuongororwa, senge yakazara ropa kuverenga (CBCS), hemoglobin ongororo, uye yeropa morphology bvunzo. Edta anochengetedza cellular zvikamu zveropa, achizviita kuti zviite bvunzo dzinoda maseru eropa, dzakadai seyechena eropa masero eropa uye matsvuku eropa indices.
C. Sodium Citrate machubhu
Sodium Citrate machubhu ane sodium citrate, anticoagulant iyo inosungira calcium ion uye inodzivirira kuwanda kweropa nekunyadzisa iyo clotting cascade. Aya machubhu anowanzoshandiswa kuongororwa kwezana, kusanganisira prothrombin nguva (PT), yakagadziriswa chikamu Thromboplastin nguva (Aptt), uye COLOGULY PROTOR). Sodium Citrate inochengeta ropa mune mvura yemvura, ichibvumira kuyera kwakakwana kwekuvhara nguva uye kuongororwa kweCoagation basa.
D. Heparin machubhu
Heparin machubhu ane anticoagulant Heparin, ayo anoita nekuvandudza basa reanti iii, inhibitor yeiyo thrombin uye zvimwe zvinhu zvinovhara. Aya machubhu anoshandiswa ezvechokwadi emakomduketi eCemisties, akadai seplasma Amonia mazoni, zvimwe zvine chekuita necopelogy ass, uye kuridza zvinodhaka zvinodhaka. Heparin inthbits coagulation nekuita zvakarerekera kurira uye kudzivirira fibrin kuumbwa, zvichiita kuti ive yakakodzera bvunzo dzinoda samples yemahara magirazi ekuvhara.
E. Fluoride oxalate tubes
Fluoride oxalate tubes ane sodium fluoride uye potassium oxalate, iyo inoshanda seanoshanda-antiglytic vamiririri vekuInhibit glycolys samples. Aya machubhu anonyanya kushandiswa kuongororwa kweGlucose, sezvo glycolysis inogona kutungamira kuderera kweganhuzi yeglucose nekufamba kwenguva. Sodium Fluoride inodzivirira kuputsa kwe enzymatic yeGlucose, nepo potasasium oxcalate inoshanda seinochengetedza. Fluoride oxalate materu akakosha kune glucose kuongororwa kweGlucelance, chirwere cheshuga cheshuga, uye kuongorora glycemic kutonga mune varwere vane chirwere cheshuga.
F. Glycolytic inhibitor machubhu
Glycolytic inhibitor machubhu ane zvinowedzera izvo inhibit glycolysis, iyo metabolic nzira ine mhosva yekuparara kweglucose. Aya machubhu anoshandiswa kudzivirira kusvibiswa kweiyo enzymatic ye glucose muropa samples, kuona zviyero zvakaringana uye zvakavimbika zveGlucose nekufamba kwenguva. Glycolytic inhibitor inokosha kune bvunzo dzinoda kuongororwa kwakagadziriswa glucose mazinga, senge glucese kutsungirira bvunzo, insulin kuramba kuongororwa, uye chirwere cheshuga maneja. Zvakawanda zvekuwedzera zvinosanganisira sodium fluoride, potassium oxacolate, uye sodiced
III. Misiyano muTube kuumbwa uye Kuwedzera
Imwe neimwe mhando yeropa yekuunganidza tube ine zvakanyanyisa zvekuwedzera zvakagadzirirwa kuchengetedza zvikamu zveropa uye inhibit isingadiwe biochemical maitiro. Kunzwisisa misiyano iyi kwakakosha pakusarudza iyo yakakodzera tube kune imwe neimwe clinical application.
Iv. Clinical application uye inoshandisa
A. Serum inoparadzanisa machubhu (sst)
STT Tubes ine glas inoparadzanisa iyo inoparadzanisa serum kubva kuRopa rese pagungano. Ivo vanowanzo shandiswa pamakemikari bvunzo, kusanganisira chiropa basa bvunzo, video profiles, uye zviyero zvemagetsi.
B. Ethylynediamaminyacetactic acid (Edta) machubhu
Edta machubhu ane Edta, anoridza mhere inosungira calcium ion uye inodzivirira ropa kuvhara kweropa nekukanganisa kabhokisi. Ivo vanoshandiswa kune hematology bvunzo, dzakadai seiro yeropa kuverenga (CBC) uye yeropa morphology bvunzo.
C. Sodium Citrate machubhu
Sodium citrate machubhu ane sodium citrate, inoita senge anticoagulant nekusunga calcium ion uye kudzivirira ropa clot yekuumbwa. Ivo vanoshandiswa bvunzo dzekuverenga, kusanganisira prothrombin nguva (PT) uye yakagadziriswa chikamu che thrombplastin nguva (APTT).
D. Heparin machubhu
Heparin machubhu ane Heparin, anenge ane simba anonyanya kufananidza iyo inhibits thrombin uye chokwadi Xa mune coagulation cascade. Ivo vanoshandiswa kune inokosha kemikistry bvunzo, yakadai seplasma Ammonia uye zvimwe zvine chepfu masaini.
E. Fluoride oxalate tubes
Fluoride oxalate tubes ane sodium fluoride uye potassium oxalate, iyo inhibit glycoling uye chengetedza glucose mazinga emasampuli epa samples. Ivo vanoshandiswa kuongororwa kweglucose, kunyanya mune chirwere cheshuga.
F. Glycolytic inhibitor machubhu
Glycolytic inhibitor machubhu ane zvinowedzera izvo inhibit glycolysis, kudzivirira kuputsa kweglucose muropa samples. Ivo vanoshandiswa kuti bvunzo dzinoda kuyerwa kwakaringana kweganhuzi dzeGlucose nekufamba kwenguva, senge glucose kushivirira bvunzo.
V. Kufunga nezvekuunganidzwa kweropa uye kubata
Maitiro akakodzera ekuunganidza ropa, kubata, uye kuchengetedza kwakakosha kuti uve nechokwadi chekuvimbika kwetsananguro yeropa uye kurongeka kwemhedzisiro yemiedzo. Pre-ongororo-aurosties, yakadai sekusvibiswa kwemuenzaniso uye hemolysis, inogona kubatsira zvakanyanya zvibodzwa zvinobatsira uye zvinofanirwa kuderedzwa kuburikidza nekuomerera kumhepo yakatanga protocol.
Vi. Runako Rudzi uye Zviitiko
Kufambira mberi muropa rekuunganidza Taguji tekinoroji inoramba ichivandudza kushanda uye kuvimbika kwekuongorora kwekuongorora. Anobuda matekinoroji, akadai se microfluidic zvishandiso uye point-of-yekuongorora mapuratifomu ekukurumidza uye akakwenenzverwa reropa rekuongorora, anovandudza kuchengetedza kwemurwere uye nekiriniki yekurarama.
Mukupedzisa kweropa, madhiri ekuunganidza eropa anotamba basa rakakosha mune razvino hutano nekugonesa kuongororwa kwakaringana uye kwakavimbika kwemasangano eropa ezvinangwa zvekuongorora. Vanonzwisisa mhando dzakasiyana dzemabhuru, manyorerwo avo, uye maClinical mashandiro akakosha kune vehutano nyanzvi vanobatanidzwa mukufungidzira, kuongororwa kwemarabhoritari, uye kutarisirwa kwemurombo. Nekutevera maitiro akanakisa ekuunganidza ropa uye kubata uye kugara uchiziviswa nezve kufambira mberi mune tekinoroji, vagari vehutano vanogona kuve nechokwadi chekutakura kwemhando yepamusoro uye nemhedzisiro yemurwere.
