UV-vis bashebelli ba sebelisoa haholo libakeng tse fapaneng tsa saense. Leha ho ne ho le bohlokoa, batho ba bangata ha ba utloisise ka botlalo hore na bolomi bona ke eng, likopo tsa bona le hore na li sebetsa joang. Sengoliloeng sena se ikemiselitse ho fana ka tlhaloso e tebileng ea UV-Prey SpectroPhotometers, e akaretsang melao ea tsona, e sebelisa, le maemo ao a sebetsang ho ona.
UV-vic spectortometer ke eng?
UV-vis spectrophotometer ke sesebelisoa sa tlhahlobo se sebelisitsoeng ho lekanya botebo ba leseli (UV) le Spectus ea Spectrum ea Specturmu. Liletsa tsena ke tsa bohlokoa ho sekaseka thepa ea mahlo, e khethollang ho tsepamisa maikutlo le ho utloisisa boits'oaro ba bona tlas'a maemo a bobebe a bobebe.
UV-vic specphetometer e kae?
Ts'ebetso ea karolo ea UV-Prey Spectrophotometer e kenyelletsa likarolo tse 'maloa tsa bohlokoa le mehato:
Mohloli oa Leseli:
SpecSORRetometer e na le mohloli oa leseli, hangata e kopantsoeng ea lebone la Deturium (bakeng sa Leseli la UV) le lebone le bonahalang le bonahalitsoeng). Makhoba ana a emisa lebone ka mose ho o bala le uena.
Monochromator:
Leseli le hlahisitsoeng ke mohloli le feta ka har'a monochromaiti e le e arohanyang ho isa qubung ea ka kotloloho. Hangata sena se etsoa ho sebelisa ho ba le tšireletso kapa ho fapana hoa grating.
Sampole ea mohlala:
Leseli la monochromatic le lebisitsoe ho sampole ea nang le sampole, moo tharollo ea sampole e kentsoeng ka har'a cuvette, e entsoe ka khalase kapa quart.
Seteishene:
Kamora ho feta ka sampole, leseli le fihla mopoliti. Sesebelisoa sa tlolo se lekanya botebo ba leseli le fetisitsoeng ebe o o fetola lets'oao la motlakase.
Tlhahlobo ea data:
Letšoao la motlakase le sebetsoa ka k'homphieutha kapa Microprocussor, e hlahisang sebopeho se bonts'ang ho ba sieo kapa ho fetisoa ha mohlala.
Melao-motheo ea UV-Vinna Spectophotometry
Molao-motheo oa mantlha oa UV - Press Spectrophotometry ke molao oa Beer-lambert, a amanang le ho monya leseli mabapi le hore leseli le lule le lona. Molao o hlahisoa e le:
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Moo:
A ke ho ahele (ho se na likarolo, joalo ka karolelano).
Na li-harbforpticgraphy e sa sebetseng hantle (L / Molhal), khafetsa tse bonts'ang leseli le monya lebone ka tsela e itseng.
ke khatello ea mefuta e monyang ea sampole (mol / L).
ke bolelele ba tsela moo leseli le tsamaeang ho sampole (cm).
Ho kena ka mokhoa o lekanang ka kotloloho bolelele ba bophahamo le tsela, ho etsa UV-Prey Spectrophotometry bakeng sa tlhahlobo ea bongata.
Likopo tsa UV-Vickis Spectrostroesters
UV-vis li-arectrophotometers li na le likopo tse fapaneng tsa likopo tse fapaneng:
1. Chemistry
Boikemisetso ba mahloriso:
Uv-vis balepetoments ba tloaetse ho ngola hangata ho lekola khatello ea maikutlo mabapi le tharollo. Mohlala, ho tsepamisa mohopolo ha DNA, liprotheine kapa liprotheine kapa liprotheine tse ling li ka lekanngoa ke ho anyesalisoa ha tsona ka li-waveleng tse ikhethang.
Reaction Kintics:
Liletsa tsena li thusa ho ithuta litefiso tsa liphetoho tsa lik'hemik'hale ka ho lekola phetoho ea ho amohela phetoho ea ho amohela phetoho ea ho amohela phetoho kapa lihlahisoa ka nako.
Tlhahlobo ea lik'hemik'hale:
Li sebelisoa bakeng sa tlhahlobo ea lik'hemik'hale, e thusang ho khetholla lintho tse thehiloeng ho Spectabiwas.
2. Boachemistrist le biogy ea biolocular
Protheine le nyutledi a nyutledic acid:
Uv-vis spectrophotometry e bohlokoa ho biochemist bakeng sa ho lekanya mahloriso le bohloeki ba acid ea nyutlelie (DNA le Rna) le liprotheine.
Ts'ebetso ea enzyme:
Ts'ebetso ea li-enzyme e ka ithutoa ka ho lekanya ho ba sieo ha likarolo kapa lihlahisoa tse kenyelletsang karabelo ea enzzzyatic.
3. Saense ea tikoloho
Teko ea boleng ba metsi:
UV-Vickis tse sirelelitsoeng ho bona le ho khetholla litšila ka metsing, joalo ka niterate, phosphates le tšepe e boima.
Ho beha leihlo la moea:
Ba thusa ho hlokomela litšila tsa moea ka ho lekanya ho ahele ha likhase tse kang Ozone le naetrogen Dioxide.
4. Tlhahlobo ea li-gralinical le phalliso
Teko ea Lithethefatsi le Nts'etsopele:
Indastering ea meriana, UV-Per - Press Spectrostotoments e sebelisetsoa ho sekaseka litlhare le bohloeki ba lithethefatsi le ho ithuta metsoako ea metsoako le ho ithuta metsoako ea metsoako e mengata.
LITLHAKISO TSA BOPHELO:
Lisebelisoa tse joalo li thusa ho li-clinikical ka ho lekanya litšitiso tse fapaneng tsa lintho tse fapaneng ho maro a 'mele, joalo ka Glucose le Bilirubin.
5. Liikela tsa Lijo le tse Khethehileng
Taolo ea Boleng:
UV-Vicki ea spectrophotometry e sebelisoa ho netefatsa hore boleng le polokeho ea lijo le liphokojoe ka ho lekanya le ho lekanya le ho lekanya mahloriso, a bolokiloeng le lithipa.
Tlhahlobo ea phepo e nepahetseng:
Khatello ea livithamini, liminerale le lihlahisoa tse ling tsa limatlafatsi tsa lijo li ka ikemisetsa ho sebelisa mokhoa ona.
Mefuta ea UV-Vind SpectroPoeters
UV-vis spectrosrophotometers co
'na ka har'a mathata a fapaneng, e' ngoe le e 'ngoe e tšoaneletsoa lits'ebetsong tse tobileng:
Setsi se le seng sa Single-Beam
Tsena li na le tsela e le 'ngoe e bobebe, e bolelang hore litabeng tsa sampole li nkoa ka tatellano. Li bonolo ebile li theko e boima haholo empa li ka ba se nepahetseng ka lebaka la ho feto-fetoha le maemo a matla a matla mohloling o bobebe.
Li-spectrophotometers tsa habeli:
Lisebelisoa tsena li arola leseli litseleng tse peli, e le 'ngoe e feta sampole le e' ngoe ho latela. Sethaleng sena se lumella tekanyo e ka thoko, e letsetsang bakeng sa ho feto-fetoha ha maemo ka tekanyo e bobebe le ho fana ka litholoana tse nepahetseng haholoanyane.
Babali ba Microroplate:
E etselitsoe ho hlahlobisisa ho feta, babali ba Microrolate ba ka lekanya li-sampole tse ngata ka nako e ngata, hangata ba sebelisoa li-biotechnology le indasteri ea meriana.
UV e nkehang ea UV ea UV
Likhatiso tsena, lisebelisoa tsa letsoho li sebelisoa bakeng sa mosebetsi oa masimong le tlhahlobo ea marang-rang, li fana ka mokhoa o bonolo oa taolo le maemo a taolo ea tikoloho le maemo.
Mekhoa e tsoetseng pele le mefuta
UV-vis spectrophotometry e bile teng ho kenyelletsa mekhoa e tsoetseng pele:
1. Derivative Spectrophotometry
Mokhoa ona o hloka ho bala matla a ho abua, ho ntlafatsa bothata ba litlhōrōpo tse fetang le ho ntlafatsa ho nepahala ha litekanyo tse rarahaneng.
2.-Flow-flow-flow-flow spectrophotometry
E ne e ithuta li-kinetics tse potlakileng tse ntseng li sebetsa, li tsamaisana le thepa e ileng ea emisa ka potlako le ho beha mabaka khafetsa, ho fana ka leseli tsamaisong ea bokhabane le lik'hemik'hale.
3. Photosustic Spectroscopy
Mokhoa ona o lekanya maqhubu a molumo a hlahisoang ke ho monya leseli, ho fana ka maikutlo a phahameng bakeng sa ho ithuta ka thata le operaquetry e kanna ea se sebetse.
Melemo le mefokolo
Melemo
E se nang kotsi:
UV-Vinnak SpeckhatotoMerry ka kakaretso ha e senyehe, e boloka mohlala bakeng sa tlhahlobo e 'ngoe.
Bonsiti e phahameng le kholiseho:
Mokhoa o fana ka kutloelo-leseling e phahameng le ho nepahala, ho etsa hore e be e loketseng ho lemoha le ho hlahisa methapo e tlase ea lihlahlobo.
Ho fapanyetsana:
E ka sekaseka lintho tse ngata haholo, ho kopanyelletsa le metsoako e tsoang le e tsoang le le leng le kahare (le tiileng le khase).
E potlakileng ebile e bonolo:
Litakatso hangata li potlakile, li lumella tlhahlobo e sebetsang le e tloaelehileng.
Meeli
Likhatiso:
Ho ba teng ha lintho tse hokahaneng tse kenang ka li-waveleth tse tšoanang li ka baka tlhahlobo eo.
Mohlala:
Sampole tse ling li ka hloka ho itukisetsa ho phatloha kapa ho khutsisa haholo ka ho hlahisa liphoso.
TLHOKOMELISO E FUMANENG:
UV-vis spectrophotometry e fana ka tlhahisoleseling haholo mabapi le ho tsepamisa maikutlo le ho ba teng liteketeng tse tlatselletsang tse kang boima ba ho kenyelletsa le nmr.
UV-Prey SpectroPototomen ke lisebelisoa tse sa tloaelehang tsa mahlale a sejoale-joale, ho fana ka mokhoa o ikhethileng le o matla oa ho sekaseka lintho tse ngata tse ngata. Likopo tsa tsona tsa likopo tse hole le masimo a fapaneng, ho kopanyelletsa le k'hemistri, mahlale a tikoloho, mahlale a bongaka le indasteri ea mela. Ho utloisisa melao-motheo, ts'ebetso le tšebeliso ea UV-PRES SHITYROCHOPHOPHOPHOPHOPHOPHOMphotometry Leha ho ne ho e-na le mefokolo ea eona, UV-Perroprophotometer e ntse e le lejoe le lejoe la laboratories la ka ha ho beleha.
