Nke onye na-agụ elisa bụ immunoassay nwere njikọ enzyme.Ọ bụ ngwá ọrụ pụrụ iche maka nyocha nke immunosorbent jikọtara enzyme, nke a na-akpọkwa microplate detector.Enwere ike kewaa ya naanị ụzọ abụọ: ọkara akpaka na akpaka zuru oke, mana ụkpụrụ ọrụ ha bụ otu.Isi ihe bụ agba agba, na okwu ndị ọzọ, a na-eji nyocha agba agba.Mkpebi ahụ n'ozuzu na-achọ ka olu ikpeazụ nke ngwọta ule dị ihe na-erughị 250μL, na ule ahụ enweghị ike ịmecha ya na mkpokọta fotoelectric colorimeter, ya mere enwere ihe pụrụ iche chọrọ maka photoelectric colorimeter na onye na-agụ elisa.