Oseihe nwoke Agụ akwụkwọ Elisa bụ ihe ejikọtara enzyme - jikọtara. Ọ bụ ngwa pụrụ iche maka ọgwụ mgbochi Enzyme - Njikwa nchọpụta microplate nchọpụta. Enwere ike kewaa ya ụzọ abụọ: ọkara akpaka na akpaka, mana ụkpụrụ ọrụ ha bụ otu. Isi ya bụ ihe na-acha odo odo, na okwu ndị ọzọ, nyocha a na-acha odo odo na-eji. Mkpebi nke a na-achọkarị nke ikpeazụ nke usoro nnwale nke ihe na-erughị 250μL, ya mere, enwere ihe pụrụ iche foto) Elisa Reader.