ʻO ka ʻO elisa heluhelu ka immunoassay pili i ka enzyme. He mea hana kūikawā ia no ka ho'āʻo immunosorbent e pili ana i ka enzyme, i kapa ʻia ʻo microplate detector. Hiki ke hoʻokaʻawale wale ʻia i ʻelua mau ʻāpana: semi-aunoa a piha piha, akā ʻo kā lākou mau loina hana he like. ʻO ke kumu he colorimeter, ʻo ia hoʻi, hoʻohana ʻia ka loiloi colorimetric. Pono ka hoʻoholo ʻana i ka nui hope o ka hopena hoʻāʻo ma lalo o 250μL, a ʻaʻole hiki ke hoʻopau ʻia ka hoʻāʻo me ka colorimeter photoelectric maʻamau, no laila aia nā koi kūikawā no ka colorimeter photoelectric i ka elisa heluhelu.