UV-vis Sportrophhotometers ndi zida zamakono zomwe zimagwiritsidwa ntchito kwambiri m'minda yasayansi yasayansi. Ngakhale kuti anthu ambiri samvetsetsa bwino zomwe zida izi zili, zomwe amagwiritsa ntchito, komanso momwe amagwirira ntchito. Nkhaniyi ikufuna kupereka chidziwitso chakuya cha UV-vispebolaphhotometers, kuphimba mfundo zake, ndi zochitika zomwe amalemba ntchito.
Kodi UV-vis Spectophotorometer?
A UV-WhertOphoroPhotometer ndi chipangizo chowunikira chomwe chimagwiritsidwa ntchito kuyeza kukula kwa kuwala mu ultraviolet (UV) ndi zigawo (mas) zamagetsi mawonekedwe. Zidazi ndizofunikira kwambiri pakusanthula zotsatsa za zinthu zomwe zimapangitsa kuti aziganizira, komanso kumvetsetsa momwe amakhalira mosiyanasiyana mosiyanasiyana.
Kodi ntchito ya UV-IY-IY-WYIPHomerometerometer imagwira bwanji?
Kachitidwe ka UV-WhopePophorotometer imaphatikizapo zinthu zingapo zazikulu:
Gwero La Kuwala:
The Spectrophotometer imakhala ndi gwero lowunikira, kuphatikiza mu nyali za deutikhwiri (kwa kuwala kwa UV) ndi nyali yowoneka (yowoneka bwino). Magetsi awa amawunikira kudutsa uV ndi mawonekedwe owoneka.
Monochromator:
Kuwala komwe kunadziwika ndi gwero kumadutsa mu monochromator, omwe amalekanitsa kukhala mawonekedwe amodzi. Izi nthawi zambiri zimachitika pogwiritsa ntchito prism kapena kuphatikiza kosiyanasiyana.
SAMPLE SARD:
Kuwala kwa monochromatic kumangirizidwa kudzera pa kambuku, pomwe zitsanzo zothetsera ziphuphu zimayikidwa mu cuvette, chidebe chaching'ono chopangidwa ndigalasi kapena quartz.
Chonyansa:
Atadutsa kudzera pa zitsanzo, kuwalako kumafika. Chojambulacho chimayesa kukula kwa kuwala kopakidwa ndikusintha kukhala chizindikiro chamagetsi.
Kusanthula kwa Data:
Chizindikiro cha magetsi chimakonzedwa ndi kompyuta kapena microprocesser, omwe amapanga mawonekedwe owoneka bwino kapena kupatsirana kwa zitsanzozo pamiyendo yosiyanasiyana.
Mfundo za UV-IY Plactophotometry
Mfundo yofunika kwambiri yaku UV-Ily Sportrophirotometry ndiye lamulo la Beer-lambirt, lomwe limafotokoza za kuyamwa kwa kuwala kwa zinthu zomwe kuunikaku komwe kuunikaku ukuwunikira. Lamulo limafotokozedwa monga:
= ⋅⋅
kuti:
Njira ndi yotsekemera (palibe mayunitsi, chifukwa ndi gawo).
Kodi kuyamwa kwa Molar ndi koema (L / Mol cmm), kosalekeza komwe kumawonetsa momwe zinthu zimathandizira kuti zinthu zikhale mwamphamvu kwambiri.
ndi kuchuluka kwa mitundu yolumikizira mu zitsanzo (mol / l).
Ndi kutalika komwe kuwala kumayenda mu zitsanzo (cm).
Kuyamwa kumakhala koyenera mwachindunji pamtunda wa kuchuluka ndi kutalika kwake, ndikupanga UV-vis Sportrophototry chida champhamvu pakuwunika kochulukirapo.
Mapulogalamu a UV-Ty Ponecrophotometers
UV-vis Sportrophhotometers ali ndi ntchito zosiyanasiyana m'minda yosiyanasiyana:
1. Chemistry
Kukhazikika Kwambiri:
UV-vis spectophotondometers amagwiritsidwa ntchito mokwanira kuti mudziwe kuchuluka kwa zosungunulira mu yankho. Mwachitsanzo, ndende ya DNA, mapuloteni, kapena biomoleclecles ina imatha kuyesedwa ndi kutengeka kwawo kumanda.
Kuchita Knitics:
Zidazi zimathandizira pakuphunzira mitundu ya zochita za mankhwala powunikira kusintha kwa zowonjezera kapena zogulitsa pakapita nthawi.
Kusanthula Kwachizindikiro:
Amagwiritsidwa ntchito popenda ndi kuchuluka kwa mankhwala opanga mankhwala, kuthandiza kuzindikira zinthu malinga ndi momwe amawonera.
2.
Mapuloteni ndi ma nucleac acid sequication:
UV-vis Spoctophotometry ndizofunikira mu biocheminry yoyezera ndende ndi kuyera kwa ma acid a acid (DNA ndi RNA) ndi mapuloteni.
Ntchito ya Enzyme:
Ntchito za ma enzymes zitha kuphunziridwa ndikuyeza kuyamwa kwa magawo kapena zinthu zomwe zimakhudzidwa ndi enzymatic zimachitika.
3. Sayansi yachilengedwe
Madzi oyesa bwino:
UV-vis Sportrophhotometers amagwiritsidwa ntchito kuzindikira ndi kupanga zodetsa m'madzi, monga ma nitrate, ma phospha, ndi zitsulo zolemera.
Kuwunika kwa mpweya:
Amathandizira powunikira zodetsa zoipitsa poyeza kuyamwa kwa mpweya ngati ozone ndi nayitrogeni dioxide.
4. Kusanthula kwamankhwala ndi mankhwala
Kuyesa Mankhwala ndi Kukula:
M'makampani opanga mankhwala, UV-vis-vis-visy amagwiritsidwa ntchito posanthula kuchuluka kwa mankhwalawa komanso kuyera kwa mankhwala osokoneza bongo komanso kusanja bata ndi kuwonongeka kwa mankhwala opangira mankhwala.
Kuzindikira Mankhwala:
Zidazi zimathandizira kuzindikira matenda azachipatala powona zinthu zosiyanasiyana zowonjezera zomwe zimamwa madzi ambiri, monga shuga, cholesterol, ndi Bilirul.
5.. Zakudya ndi Zakudya zakumwa
Kuwongolera kwapadera:
UV-vis Sportrophirotometry imagwiritsidwa ntchito kuonetsetsa kuti chakudya ndi chodzitchinjiriza ndi zakumwa zoyezera kuchuluka kwa zowonjezera, zoteteza, ndi zodetsa nkhawa.
Kusanthula Kwathunthu:
Kukhazikika kwa mavitamini, michere, ndi michere ina yazakudya zimatha kutsimikiza pogwiritsa ntchito njirayi.
Mitundu ya UV-WOSPOCOMBOTOTOTOTOTOTOTOTOTOTOTOTOMETOMOTETETETERSH
UV-vis Sportrophotometer Co
Ine mosintha zosiyanasiyana, chilichonse chimayenererana ndi mapulogalamu apadera:
Mtengo wokhawo wokhathanthu:
Awa ali ndi njira imodzi yowala, kutanthauza kuti owerengera komanso magawo zitsanzo amagwiritsidwa ntchito motsatira. Ndizosavuta komanso zotsika mtengo koma zimakhala zosalondola chifukwa cha kusinthasintha kwa kusintha kwamphamvu.
Mtengo wowerengeka wowirikiza kawiri:
Zidazi zimagawanitsa kuwala kwa njira ziwiri, imodzi yomwe ikudutsa mwachitsanzo ndi ina kudzera mu zonena. Kukhazikitsa kumeneku kumalola muyeso munthawi yomweyo, kulipirira kusinthasintha mwachidule komanso kupereka zotsatira zolondola.
Owerenga Micreete:
Zopangidwira kuwunikira kwambiri, owerenga maikolofoni amatha kuyesa mitundu yambiri pogwiritsa ntchito miteyo yokhala ndi zitsime zingapo, zomwe zimagwiritsidwa ntchito mu biotechnology ndi mafakitale opangira mankhwala.
UV-vis Sportrophotometers:
Zida zophatikizika, zoyamika zimagwiritsidwa ntchito pofuna kuwongolera zojambulajambula komanso kusanthula kwa malo, kupereka zosavuta komanso kusinthasintha kwa kuwongolera kwa chilengedwe ndi mtundu wapadera.
Njira Zapamwamba ndi Kusintha
UV-vis Sportrophototrometry yasintha kuphatikiza njira zotsogola ndi kusiyanasiyana:
1.
Njira iyi imaphatikizapo kuwerengera kuti kuphatikizika kwa mawonekedwe a spectrum, kumapangitsa kuthetsa kwa nsonga zokulira ndikuwongolera kulondola kwa magawo osokoneza bongo omwe amasinthana.
2. Kuyimitsidwa
Ankakonda kuphunzira mwachangu katswiri wa Kainikrophototry imasakanikirana mwachangu ndikuyesa kudziwitsa njira zosinthira mu njira zamankhwala komanso mankhwala.
3.. Phokoso
Njirayi imayesa mafunde a mawu opangidwa ndi kuwala kosinthika kwa kuwala kosinthika, kupereka chidwi kwambiri cha zitsanzo zolimba ndi opaque pomwe zikhalidwe UV sizingakhale zothandiza.
Ubwino ndi Zofooka
Ubwino
Zowononga:
UV-vis Sportrophirotometry nthawi zambiri siowononga, kusunga zitsanzo kuti zisanthulidwenso.
Chidwi chachikulu komanso molondola:
Njirayi imapereka chidwi kwambiri komanso kuwongolera, kupangitsa kukhala koyenera kuzindikira ndi kumatha kumatha kuchepa kwambiri.
Kusiyanitsa:
Itha kusanthula zinthu zingapo, kuphatikizapo mankhwala opangidwa ndi organic, m'malo osiyanasiyana (olimba, amadzimadzi, ndi mpweya).
Mwachangu komanso zosavuta:
Miyeso nthawi zambiri imafulumira komanso yolunjika, kulola kusanthula koyenera komanso kosalekeza.
Kuchepetsa malire
Zosokoneza:
Kukhalapo kwa zinthu zomwe zimatenga komwe kumayendanso kumatha kusokoneza kusanthula.
Kukonzekera zitsanzo:
Zitsanzo zina zimafuna kukonzekera kwakukulu kapena kusamala, komwe kumatha kuyambitsa zolakwika.
Zambiri zochepa:
UV-vis Sportrophototry makamaka imapereka chidziwitso pazomwe zimachitika komanso kuphatikiza mitundu koma imasowa mwatsatanetsatane, zomwe zimafuna njira zokwanira monga spectmetry kapena nmr.
UV-vis Spocraphhotometers ndi zida zofunikira kwambiri mu sayansi yamakono, ndikupereka njira yosiyanasiyana komanso yamphamvu yopenda zinthu zingapo. Mapulogalamu awo anali atangokhalira kudutsa mitundu yosiyanasiyana, kuphatikiza chemistry, biochemistry, sayansi yachilengedwe, matenda azachipatala, komanso malonda. Kuzindikira mfundo, opareshoni, ndi kugwiritsa ntchito kwa UV Sportrophotopotometry imalola asayansi ndi akatswiri kuti azitha kufufuza ndi chitukuko, komanso zolinga zapamwamba. Ngakhale kuti anali ndi malire, UV-WE-WEYIPOMOTmememememememeter ya labortolong ya ma laborator, ikuthandizira kwakukulu kwa kayendedwe ka sayansi ndi ukadaulo.
