An i-autoclave iyisisetshenziswa esisebenzisa isitimu ukuvala amathuluzi nezinye izinto.Lokhu kusho ukuthi wonke amabhaktheriya, amagciwane, isikhunta, kanye nezinhlamvu kuyabhujiswa. Ama-autoclave asebenza ngokuvumela isitimu ukuthi singene futhi sigcine umfutho ophakeme kakhulu okungenani imizuzu eyi-15.Ngenxa yokuthi kusetshenziswa ukushisa okumanzi, imikhiqizo ye-heat-labile (njengamanye amapulasitiki) ayikwazi ukuvalwa inzalo noma izoncibilika.